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Analysis of MSCs Characteristics. (A) Strategy for inducing hESCs differentiation into <t>fibroblasts</t> and constructing tissue-engineered dermal substitutes. (B) The spindle-like morphology of hESC-MSCs (a) and hMSCs (b) . Scale bar = 100 μm. (C) Flow cytometric analysis of cell surface antigen expression on hESC-MSCs (a) and hMSCs (b) . (D) Quantitative analysis of MSC cell surface markers (data represent mean ± SD, n = 3). ** p < 0.01.
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Analysis of MSCs Characteristics. (A) Strategy for inducing hESCs differentiation into fibroblasts and constructing tissue-engineered dermal substitutes. (B) The spindle-like morphology of hESC-MSCs (a) and hMSCs (b) . Scale bar = 100 μm. (C) Flow cytometric analysis of cell surface antigen expression on hESC-MSCs (a) and hMSCs (b) . (D) Quantitative analysis of MSC cell surface markers (data represent mean ± SD, n = 3). ** p < 0.01.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds

doi: 10.3389/fbioe.2026.1777175

Figure Lengend Snippet: Analysis of MSCs Characteristics. (A) Strategy for inducing hESCs differentiation into fibroblasts and constructing tissue-engineered dermal substitutes. (B) The spindle-like morphology of hESC-MSCs (a) and hMSCs (b) . Scale bar = 100 μm. (C) Flow cytometric analysis of cell surface antigen expression on hESC-MSCs (a) and hMSCs (b) . (D) Quantitative analysis of MSC cell surface markers (data represent mean ± SD, n = 3). ** p < 0.01.

Article Snippet: Human skin fibroblasts (HSF, ATCC PCS-201–012®TM) were used as a positive control.

Techniques: Expressing

Fibroblastic differentiation of hESC-MSCs. (A) Cellular morphology of hESC-Fbs and HSF. Scale bar = 100 μm. (B) Immunofluorescence analysis of VIM and CK5 expression in hESC-Fbs and HSF. VIM and CK5 were shown with green, nuclei were counterstained with blue. Scale bar = 100 μm. (C) qPCR assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). * p < 0.05 and ** p < 0.01 vs. hESC-MSCs. (D) ELISA assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). ** p < 0.01 vs. hESC-MSCs.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Tissue-engineered dermal substitutes constructed by human embryonic stem cell-derived fibroblasts facilitate the repair of skin wounds

doi: 10.3389/fbioe.2026.1777175

Figure Lengend Snippet: Fibroblastic differentiation of hESC-MSCs. (A) Cellular morphology of hESC-Fbs and HSF. Scale bar = 100 μm. (B) Immunofluorescence analysis of VIM and CK5 expression in hESC-Fbs and HSF. VIM and CK5 were shown with green, nuclei were counterstained with blue. Scale bar = 100 μm. (C) qPCR assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). * p < 0.05 and ** p < 0.01 vs. hESC-MSCs. (D) ELISA assay of fibroblast relevant markers during fibroblastic differentiation of hESC-MSCs (data represent mean ± SD, n = 3). ** p < 0.01 vs. hESC-MSCs.

Article Snippet: Human skin fibroblasts (HSF, ATCC PCS-201–012®TM) were used as a positive control.

Techniques: Immunofluorescence, Expressing, Enzyme-linked Immunosorbent Assay